Imagine unlocking the full potential of RNA sequencing, revealing hidden layers of genetic information that were once out of reach. That's exactly what RNAConnect is doing with its groundbreaking UltraMarathonRT® Direct RNA-Seq Kit. This innovative tool is set to revolutionize nanopore-based native RNA sequencing, offering researchers a deeper, more comprehensive view of the transcriptome. But here's where it gets exciting: it's not just about seeing more—it's about seeing better.
RNAConnect, a trailblazer in life science tools, has officially launched the UltraMarathonRT® Direct RNA-Seq (uMRT DRS) Kit, a game-changing solution designed for Oxford Nanopore Technologies® direct RNA sequencing (SQK-RNA004). This kit isn’t just another addition to the market; it’s a leap forward in RNA analysis. By employing a gentle 30°C reverse-transcription (RT) stabilization step and ultra-processive enzyme performance, the kit preserves RNA integrity like never before. This means researchers can detect more genes, more isoforms, and longer transcripts—all while maintaining endogenous RNA modification signals. And this is the part most people miss: it does this without compromising the native structure of the RNA, ensuring a more accurate representation of the transcriptome.
Direct RNA-Seq typically requires an initial reverse transcription step to smooth out secondary structures and stabilize the RNA before it’s pulled through the nanopore for reading. This process allows for strand-specific, isoform-resolved measurements alongside base-modification information. The UltraMarathonRT Direct RNA-Seq Kit takes this workflow to the next level with optimized reagents tailored for ONT RNA004 chemistry and a low-temperature RT step that maximizes the sequencing of each RNA sample. The result? A more complete and accurate picture of the transcriptome.
But here's where it gets controversial: While traditional methods often fall short in capturing the full spectrum of RNA complexity, the UltraMarathonRT Kit challenges the status quo. Its novel chemistry and lower RT temperature (30°C) deliver significant improvements over existing protocols. For instance, it achieves 25-50% higher cDNA yield, 5-10% longer read lengths (with dramatic differences in transcripts >2kb), 1-2% more genes detected, 15-20% more novel transcripts, and 25-30% more intron retention events—all while maintaining equivalent mapping rates and Q scores. Is this the future of RNA sequencing, or just a niche improvement? We’d love to hear your thoughts in the comments.
Dr. Jason Underwood, VP of Technology Development at RNAConnect, explains, “We identified gaps in current direct RNA-seq protocols where our expertise in RNA and the unique properties of UltraMarathonRT could significantly enhance long RNA read output. We’re thrilled to introduce this user-friendly kit, empowering the RNA science community to make groundbreaking discoveries.”
Dr. Paul Boutz, Assistant Professor at the University of Rochester School of Medicine and Dentistry, who beta-tested the uMRT DRS Kit, adds, “Long-read sequencing technologies can capture transcripts spanning kilobases, but most reverse transcriptases can’t keep up. This limitation obscured our view of alternative splicing events. UltraMarathonRT has been a game changer, seamlessly integrating into the ONT workflow and delivering exceptional results. It’s now our go-to for direct RNA sequencing.”
The UltraMarathonRT Direct RNA-Seq Kit is available now through RNAConnect and authorized distributors. For detailed specifications, chemistry compatibility, and workflow guidance, visit RNAConnect’s website.
About RNAConnect:
RNAConnect is more than just a life science tools company—it’s a pioneer in RNA analysis solutions. With a focus on genomics, transcriptomics, and molecular diagnostics, RNAConnect equips scientists with sensitive, accurate, and reliable tools to drive discoveries in rare diseases, immunology, oncology, neurobiology, and beyond. Their mission? To empower researchers to unlock the mysteries of RNA, one breakthrough at a time.
Media Contact:
Andrew Bond, PhD — Chief Commercial Officer
Tel: +1‑475-338-2120
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SOURCE RNAConnect
Thought-Provoking Question: As RNA sequencing technologies continue to evolve, how will tools like the UltraMarathonRT Kit reshape our understanding of transcriptomics? Do you think this level of precision is necessary for all research, or is it overkill for certain applications? Share your insights below!
